目的建立HPLC-MS测定金莲清热颗粒中荭草苷、牡荆苷、金丝桃苷、新芒果苷、芒果苷、知母皂苷BⅡ和知母皂苷AⅢ7种成分含量的方法。方法采用DiamonsilC₁₈柱(4.6mm×150mm，5μm);柱温30℃;乙腈-0.1%甲酸水溶液为流动相，以800μL·min^-1梯度洗脱;质谱条件:采用电喷雾离子源正负离子模式同时进行检测，多反应监测模式(MRM)用于定量测定。结果金莲清热颗粒中7种成分峰面积与浓度呈良好的线性关系，r＞0.9992;加样回收率分别为99.34%，99.28%，101.6%，101.3%，100.5%，101.9%和98.86%(n=3)。RSD值分别为1.12%，1.36%，1.57%，1.74%，1.52%，1.43%和0.92%。结论该方法简便，准确，重现性好，专属性高，可用于金莲清热颗粒的质量控制。

OBJECTIVE To develop a method for the determination of orientin,vitexin,hyperin,neomangiferin,mangiferin,timosaponin BII and timosaponin AⅢ in Jinlianqingre granules(traditional Chinese medicines) by HPLC-MS. METHODS The samples were separated on a Diamonsil C₁₈ column (4.6 mm×150 mm,5 μm ) by gradient elution using acetonitrile and 0.1% aqueous formic acid as the mobile phase at a flow rate of 800 μL·min^-1. The column temperature was 30 ℃. Multiple-reaction monitoring (MRM) scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. RESULTS Complete separation was obtained within 15 min for the seven compounds. The regression equations showed linear relationships between the peak area and content of each compound(r＞0.999 2). The average recoveries of the compounds listed above were 99.34%,99.28%,101.6%,101.3%,100.5%,101.9% and 98.86% (n=3),and the RSDs were 1.12%,1.36%,1.57%,1.74%,1.52%,1.43% and 0.92%,respectively. CONCLUSION The method is simple,accurate and highly reproducible,and can be used for the determination of seven compounds in Jinlianqingre granules.